ISFET-based biosensor to monitor sugar metabolism in bacteria

Research group: Cellular Biotechnology Year: 2008
Type of Publication: Article Keywords: Bioelectronics
Authors: Marc Castellarnau; N. Zine; J. Bausells; C. Madrid; Antonio Juárez; Josep Samitier; A. Errachid
Journal: Materials Science and Engineering: C Volume: 28
Number: 5-6 Pages: 680 - 685
ISSN: 0928-4931
Note:
MADICA 2006 Conference, Fifth Maghreb-Europe Meeting on Materials and their Applicatons for Devices and Physical, Chemical and Biological Sensors, MADICA 2006 Conference, Fifth Maghreb-Europe Meeting on Materials and their Applicatons for Devices and Physical, Chemical and Biological Sensors
Abstract:
We report the use of ion-selective field effect transistor devices (ISFETs) with an integrated pseudo-reference electrode for on-line monitoring of bacterial metabolism by monitoring of the pH variation. As a model we tested the ability of Lactobacillus strains to ferment sugars, producing lactic acid, which results in a decrease in pH in the suspension medium. We have tested and compared sugar uptake by L. sakei and a L. curvatus strains. The results obtained show that it is possible to distinguish between both types of Lactobacillus strains through their pattern of ribose uptake. The use of ISFETs represents a non-invasive methodology that can be used to monitor biological activity in a wide variety of systems.
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