Gerard Rubí, Biomaterials for Regenerative Therapies
The majority of morphogenetic and pathological processes are driven by cells responding to the surrounding matrix cues, including matrix composition, architecture, and mechanical properties. Despite the increased evidence of extracellular matrix (ECM) properties, in vitro substitutes still fail to effectively mimic the native microenvironment. In this study, we aim to develop and characterize cell-derived extracellular matrices (CDMs) obtained through a protein deposition from human mesenchymal stem cells cultured in sacrificial 3D scaffold templates of poly-lactic acid (PLA) microcarriers. Obtained decellularized CDMs closely mimic biochemical, physical, and mechanical properties of native tissues’ ECM. The produced novel CDMs, are currently tested as a 3D cell culture platform for disease modelling. This is achieved through CDMs repopulation with colorectal cancer cells and cancer associated fibroblasts (CAFs). The new 3D CDMs-cancer platform will provide an in vitro tumor model to study the cells-ECM interactions and potential therapeutic targets, to finally serve as a drug-screening platform for personalized medicine.
Ignasi Ferrer, Biomedical Signal Processing and Interpretation
The PhD discussions session will be held ONLINE at the GoToMeeting platform