BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Institute for Bioengineering of Catalonia - ECPv6.3.5//NONSGML v1.0//EN CALSCALE:GREGORIAN METHOD:PUBLISH X-WR-CALNAME:Institute for Bioengineering of Catalonia X-ORIGINAL-URL:https://ibecbarcelona.eu X-WR-CALDESC:Events for Institute for Bioengineering of Catalonia REFRESH-INTERVAL;VALUE=DURATION:PT1H X-Robots-Tag:noindex X-PUBLISHED-TTL:PT1H BEGIN:VTIMEZONE TZID:Europe/Madrid BEGIN:DAYLIGHT TZOFFSETFROM:+0100 TZOFFSETTO:+0200 TZNAME:CEST DTSTART:20190331T010000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:+0200 TZOFFSETTO:+0100 TZNAME:CET DTSTART:20191027T010000 END:STANDARD END:VTIMEZONE BEGIN:VEVENT DTSTART;TZID=Europe/Madrid:20190925T150000 DTEND;TZID=Europe/Madrid:20190925T160000 DTSTAMP:20240329T084856 CREATED:20190919T073920Z LAST-MODIFIED:20190919T073920Z UID:68331-1569423600-1569427200@ibecbarcelona.eu SUMMARY:SEMINAR Series in Development\, Regeneration and Disease DESCRIPTION:“Engineering human pluripotent stem cells for organoid applications in regenerative medicine”\nNuria Montserrat\nCatalan Institution for Research and Advanced Studies (ICREA)\, Barcelona\, Spain.\nPluripotency for Organ Regeneration\, Institute for Bioengineering of Catalonia (IBEC)\, The Barcelona Institute of Technology (BIST)\, Barcelona\, Spain.\nCentro de Investigación Biomédica en Red en Bioingeniería\, Biomateriales y Nanomedicina\, Madrid\, Spain.\nABSTRACT \nThe generation of human pluripotent stem cells (hPSCs) derived organoids is one of the biggest scientific advances in regenerative medicine. Recently\, we have demonstrated that lengthening the time that hPSCs are exposed to a threedimensional microenvironment in the presence of defined renal inductive signals we are able to generate kidney organoids that transcriptomically match secondtrimester human fetal kidneys. Furthermore\, we have recently developed a transplantation method that utilizes the chick chorioallantoic membrane to emulate a soft in vivo microenvironment further sustaining the growth and differentiation of implanted kidney organoids\, as well as providing a vascular component. \nIn order to promote the efficient generation of nephron-like structures we have also mimicked the stiffness of the in ovo chorioallantoic membrane microenvironment in vitro by fabricating compliant hydrogels. This approach showed that by mirroring both biochemical and mechanical cues during organoid generation we are able to boost renal commitment and nephron differentiation. We will also discuss recent findings on the application of different bioengineering strategies (i.e.\, including 3D biopriting and tissue engineering) for kidney organoid generation\, as well as the definition of culture conditions mimicking renal disease. Furthermore\, we will discuss how through the use of CRISPR/Cas9 we are able to introduce mutations related to kidney developmental diseases exploiting kidney organoid technology. \nShare this...FacebookPinterestTwitterLinkedin URL:https://ibecbarcelona.eu/event/seminar-series-in-development-regeneration-and-disease/ LOCATION:Felix Serratosa\, Baldiri Reixac\, 10-12\, Barcelona\, Spain CATEGORIES:Joint seminar / workshop / symposium END:VEVENT END:VCALENDAR