Xavier Trepat | Group Leader / ICREA Research Professor
Dobryna Julia Valeria Zalvidea | Senior Researcher
Juan Francisco Abenza Martínez | Postdoctoral Researcher
Manuel Gómez González | Postdoctoral Researcher
Anna Labernadie | Postdoctoral Researcher
Andrea Malandrino | Postdoctoral Researcher
Raimon Sunyer Borrell | Postdoctoral Researcher
Léo Florent Alexandre Valon | Postdoctoral Researcher
Ernest Latorre Ibars | PhD Student
Carlos Pérez González | PhD Student
Pilar Rodríguez Franco | PhD Student
Marina Uroz Marimon | PhD Student
Natalia Castro Morán | Laboratory Technician
Ariadna Marin Llaurado | Masters Student
The ability of eukaryotic cells to migrate within living organisms underlies a wide range of phenomena in health and disease.
When properly regulated, cell migration enables morphogenesis, host defense and tissue healing. When regulation fails, however, cell migration mediates devastating pathologies such as cancer, vascular disease and chronic inflammation. Our research focuses on understanding the fundamental biophysical mechanisms underlying migration both at the single cell level and at the tissue level.
Making cellular forces visible
To study cell and tissue dynamics we develop new technologies to measure physical forces at the cell-cell and cell-matrix interface. By combining these technologies with computational analysis of cell shape and velocity we obtain a full experimental characterization of epithelial dynamics during tissue growth, wound healing and cancer cell invasion.
Mechanical waves and collective cell guidance
Our new tools led to the discovery of a mechanical wave – which we called “X-wave” – that propagates through expanding cell sheets. This mechanical wave is a natural candidate to trigger mechanotransduction pathways during wound healing, morphogenesis, and collective cell invasion in cancer. We also discovered a new mechanism, plithotaxis, by which cells align their shape and migration velocity to minimize intercellular shear stresses.
Microfabrication and wound healing
Using microfabrication technologies, we designed new ways to decipher the mechanisms of wound healing. By doing so we uncovered a new understanding of how cells move and work together to close a gap in a tissue. We showed that a new mechanism applies in which cells assemble supracellular contractile arcs that compress the tissue under the wound. By combining experiments and computational modeling, we showed that contractions arising from these arcs make the wound heal in a quicker and more robust way.
Fracking epithelial layers
Epithelial sheets must be malleable enough to adopt functional shapes during morphogenesisand to quickly self-repair after damage. Yet, they must be resilient enough to ensure organ compartmentalization and to protect organisms against environmental pathogens. To study the mechanisms that regulate this fine balance between malleability and integrity we develop tools to map epithelial tension during tissue stretching. By combining these tools with computational modeling we determined the mechanisms of epithelial fracture. Intriguingly, one of such mechanisms is hydraulic fracturing or “fracking”.
Xavier Trepat, ICREA professor and group leader at IBEC, was the guest star at the first anniversary celebration of Big Vang, La Vanguardia’s online science section.
Researchers at IBEC and their collaborators at the Centre of Regenerative Medicine of Barcelona (CMR[B]) have developed a revolutionary new technique based on photoactivation (light activation), by which cells in deep tissue can activated and tracked in vivo without causing any damage.
IBEC group leader and ICREA research professor Xavier Trepat has come third in the La Vanguardia Science Award.
Scientists at the Institute for Bioengineering of Catalonia (IBEC) have revealed that, counter to previous understanding, the living cells in our bodies behave like solids rather than the liquids they are made of.
A study by the Institute for Bioengineering of Catalonia (IBEC) reveals how cells withstand breakage during the constant changes in shape and volume experienced in most biological processes.
IBEC group leader and ICREA research professor Xavier Trepat is this year’s winner of the Banc Sabadell Award for Biomedical Research for his work on understanding the fundamental biophysical mechanisms underlying cell interaction and communication.
At a press conference at the Obra Social “la Caixa”’s Palau Macaya earlier today, Xavier Trepat, group leader at the Institute for Bioengineering of Catalonia (IBEC), Enric Banda, director of the department of Science and Environment of the Obra Social ”la Caixa”, and Josep Samitier, director of IBEC, described a study published in Nature Cell Biology which sheds new light on how to control metastasis.
Researchers at the Institute for Bioengineering of Catalonia and their collaborators reveal that they’re a step closer to optimizing cells able to guide regeneration of the spine
In an article published in the journal Nature Materials, researchers at the IBEC and the UPC describe their discovery that ‘fracking’takes place in the body at a cellular level.
Researchers at IBEC reveal in a Nature Communications paper some surprising mechanics that drive epithelial gap closure in the absence of underlying layers.
Xavier Serra Picamal, formerly a PhD student in IBEC’s Integrative Cell and Tissue Dynamics group, was awarded a prestigious Ramon Margalef Prize for the best paper derived from a doctoral thesis at a ceremony at the UB last night.
Today’s news about the Integrative Cell and Tissue Dynamics group’s paper in Nature Physics has been covered in La Vanguardia.
When we think of wound healing, we normally think of wounds to our skin. But wounds happen inside the body in all sorts of tissues and organs, and can have implications in many chronic diseases such as diabetes and asthma. Wounds also favour cancer progression by providing a physical and chemical environment that promotes the invasion of malignant cells. Now, a group at the Institute for Bioengineering of Catalonia (IBEC) has found a new way to decipher the mechanisms of wound healing, and by doing so has uncovered a new understanding of how cells move and work together to close a gap in a tissue.
Integrative cell and tissue dynamics group leader Xavier Trepat is profiled in El Mundo’s ‘Innovadores’ supplement today.
The news about Xavier Trepat’s Proof of Concept grant from the ERC appears in La Vanguardia today.
This week’s press release about the Integrative Cell and Tissue Dynamics group’s work published in Nature Materials has appeared online in La Vanguardia, ABC and El Diario.
The Integrative Cell and Tissue Dynamics group starts the year with good news from the European Research Council – they’ve been awarded both a new Consolidator Grant and a Proof of Concept award.
Results from IBEC researchers and their collaborators will pave the way for better and faster wound healing, as well opening new avenues for tissue engineering of skin
The October cover of Current Opinion in Cell Biology (the reference opinion journal in cell biology, with impact factor 12) features research by IBEC’s Pere Roca and Xavier Trepat, who together with Raimon Sunyer from the UB have published a review paper in the issue, “Mechanical guidance of cell migration: lessons from chemotaxis”.
The latest cover of Nature Materials was designed by IBEC PhD student Xavier Serra and shows a real tissue segmented computationally to give the impression of a Gaudinian trencadís.
Integrative Cell and Tissue Dynamics group leader Xavier Trepat and his recent paper in Nature Cell Biology are the subject of an article in El Périodico today.
A new discovery about how cells move inside the body may provide scientists with crucial information about disease mechanisms such as the spread of cancer or the constriction of airways caused by asthma. Researchers at IBEC and Harvard School of Public Health have found that epithelial cells—the type that form a barrier between the inside and the outside of the body, such as skin cells—move in a group, propelled by forces both from within and from nearby cells to fill any spaces they encounter.
Monday’s news about the Nature Cell Biology paper ‘Chase-and-run between adjacent cell populations promotes directional collective migration’ was covered in several science and general news sites and magazines, including La Razón.
Researchers at IBEC, the University of Barcelona and their collaborators have found that cells in our bodies, when moving collectively, carry out something similar to a game of ‘tag’ to coordinate their movement in a particular direction.
Researchers have shed new light on how the cells in our bodies collectively migrate, a critical process in positive events such as embryonic development and wound healing, but which is also integral to the development of cancer.
The Integrative Cell and Tissue Dynamics group’s recent paper published in PNAS was covered in Diario Medico.
First measurements of forces driving collective cell migration unveil new principle in biology
People can be brittle, transparent, shattered, or have a heart of glass. Now these attributes seem all the more appropriate following a discovery by researchers that migrating cells in our bodies behave in a remarkably similar way to glass when it is heated and cooled.
An article by Dr. Xavier Trepat, senior researcher of IBEC´s Cellular and respiratory biomechanics group and the Department of Physiology Sciences of the University of Barcelona, contributes for the first time an experimental answer to the question of how cells move during biological processes as diverse as the development, metastasis, or regeneration of tissues.
Press coverage elsewhere
Cells flow like glass, study finds. Harvard Science Foundation
Cells guided on their journey. Nature Physics
Disorderly conduct. Harvard Medical School Focus
How growing cells move together. Harvard Science Foundation
A stretch in cells. Nature
More than lip service. Nature
In Catalan / Spanish:
Estamos hechos de vidrio. El Mundo
L’observador. RTVE, Spanish National Radio
Com actuen les forces físiques durant la migració cel·lular? Comunicacions UB
|GENESFORCEMOTION Physical Forces Driving Collective Cell Migration: from Genes to Mechanism||European Commission, ERC-StG||Xavier Trepat|
|MICROGRADIENTPAGE Micro Gradient Polyacrylamide Gels for High Throughput Electrophoresis Analysis||European Commission, ERC-PoC||Xavier Trepat|
|TensionControl: Multiscale regulation of epithelial tension||European Commission, ERC – CoG||Xavier Trepat|
|CAMVAS Coordination and migration of cells during 3D Vasculogenesis||European Commission, MARIE CURIE – IOF||Xavier Trepat|
|El mecanoma de la adhesión epitelial: mecanismos de detección, resistencia y transmisión de fuerzas intercelulares||MINECO, I+D-Investigación fundamental no orientada||Xavier Trepat|
|DUROTAXIS Mecanobiología de la durotaxis: de las células aisladas a los tejidos||MINECO, Proyectos I+D Excelencia||Xavier Trepat|
|Joint Programme_Healthy Ageing||Obra Social La Caixa||Xavier Trepat|
Przybyla, L., Lakins, J. N., Sunyer, R., Trepat, X., Weaver, V. M., (2016). Monitoring developmental force distributions in reconstituted embryonic epithelia Methods 94, 101-113
Ladoux, B., Mège, R. M., Trepat, X., (2016). Front-rear polarization by mechanical cues: From single cells to tissues Trends in Cell Biology 266, 420-433
Tekeli, I., Aujard, I., Trepat, X., Jullien, L., Raya, A., Zalvidea, D., (2016). Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation Light: Science and Applications 56, e16084
Blanchard, R., Morin, C., Malandrino, A., Vella, A., Sant, Z., Hellmich, C., (2016). Patient-specific fracture risk assessment of vertebrae: A multiscale approach coupling X-ray physics and continuum micromechanics International Journal for Numerical Methods in Biomedical Engineering ,
Plutoni, C., Bazellieres, E., Le Borgne-Rochet, M., Comunale, F., Brugues, A., Séveno, M., Planchon, D., Thuault, S., Morin, N., Bodin, S., Trepat, X., Gauthier-Rouvière, C., (2016). P-cadherin promotes collective cell migration via a Cdc42-mediated increase in mechanical forces Journal of Cell Biology 2122, 199-217
Casares, L., Vincent, R., Zalvidea, D., Campillo, N., Navajas, D., Arroyo, M., Trepat, X., (2015). Hydraulic fracture during epithelial stretching Nature Materials 143, 343-351
Bazellières, Elsa, Conte, Vito, Elosegui, Alberto, Serra-Picamal, Xavier, Bintanel-Morcillo, María, Roca-Cusachs, Pere, Muñoz, José J., Sales-Pardo, Marta, Guimerà, Roger, Trepat, Xavier, (2015). Control of cell-cell forces and collective cell dynamics by the intercellular adhesome Nature Cell Biology 174, 409-420
Ravasio, Andrea, Cheddadi, Ibrahim, Chen, Tianchi, Pereira, Telmo, Ong, Hui Ting, Bertocchi, Cristina, Brugues, Agusti, Jacinto, Antonio, Kabla, Alexandre J., Toyama, Yusuke, Trepat, Xavier, Gov, Nir, Neves de Almeida, Luis, Ladoux, Benoit, (2015). Gap geometry dictates epithelial closure efficiency Nature Communications 6, 7683
Vedula, Sri Ram Krishna, Peyret, Grégoire, Cheddadi, Ibrahim, Chen, Tianchi, Brugués, Agustí, Hirata, Hiroaki, Lopez-Menendez, Horacio, Toyama, Yusuke, Neves de Almeida, Luis, Trepat, Xavier, Lim, Chwee Teck, Ladoux, Benoit, (2015). Mechanics of epithelial closure over non-adherent environments Nature Communications 6, 6111
Kosmalska, A. J., Casares, L., Elosegui, A., Thottacherry, J. J., Moreno-Vicente, R., González-Tarragó, V., Del Pozo, M. Ã, Mayor, S., Arroyo, M., Navajas, D., Trepat, X., Gauthier, N. C., Roca-Cusachs, P., (2015). Physical principles of membrane remodelling during cell mechanoadaptation Nature Communications 6, 7292
Vincent, Romaric, Bazellières, Elsa, Pérez-González, Carlos, Uroz, Marina, Serra-Picamal, Xavier, Trepat, Xavier, (2015). Active tensile modulus of an epithelial monolayer Physical Review Letters 11524, 248103
Lucantonio, Alessandro, Noselli, Giovanni, Trepat, Xavier, DeSimone, Antonio, Arroyo, Marino, (2015). Hydraulic fracture and toughening of a brittle layer bonded to a hydrogel Physical Review Letters 11518, 188105
Brask, J. B., Singla-Buxarrais, G., Uroz, M., Vincent, R., Trepat, X., (2015). Compressed sensing traction force microscopy Acta Biomaterialia 26, 286-294
Reginensi, Diego, Carulla, Patricia, Nocentini, Sara, Seira, Oscar, Serra-Picamal, Xavier, Torres-Espín, Abel, Matamoros-Angles, Andreu, Gavín, Rosalina, Moreno-Flores, María Teresa, Wandosell, Francisco, Samitier, Josep, Trepat, Xavier, Navarro, Xavier, del Río, José Antonio, (2015). Increased migration of olfactory ensheathing cells secreting the Nogo receptor ectodomain over inhibitory substrates and lesioned spinal cord Cellular and Molecular Life Sciences 7214, 2719-2737
García, S., Sunyer, R., Olivares, A., Noailly, J., Atencia, J., Trepat, X., (2015). Generation of stable orthogonal gradients of chemical concentration and substrate stiffness in a microfluidic device Lab on a Chip 1512, 2606-2614
Vizoso, Miguel, Puig, Marta, Carmona, F. Javier, Maqueda, Maria, Velásquez, Adriana, Gomez, Antonio, Labernadie, Anna, Lugo, Roberto, Gabasa, Marta, Rigat-Brugarolas, Luis G., Trepat, Xavier, Ramírez, Jose, Reguart, Noemí, Moran, Sebastian, Vidal, Enrique, Perera, Alexandre, Esteller, Manel, Alcaraz, Jordi, (2015). Aberrant DNA methylation in Non Small Cell Lung Cancer associated fibroblasts Carcinogenesis 3212, 1453-1463
Mrkonji, Garcia-Elias, A., Pardo-Pastor, C., Bazellières, E., Trepat, X., Vriens, J., Ghosh, D., Voets, T., Vicente, R., Valverde, M. A., (2015). TRPV4 participates in the establishment of trailing adhesions and directional persistence of migrating cells Pflugers Archiv European Journal of Physiology 46710, 2107-2119
Zaritsky, Assaf, Welf, Erik S., Tseng, Yun-Yu, Angeles Rabadán, M., Serra-Picamal, Xavier, Trepat, Xavier, Danuser, Gaudenz, (2015). Seeds of locally aligned motion and stress coordinate a collective cell migration Biophysical Journal 10912, 2492-2500
Perrault, Cecile, Brugues, Agusti, Bazellieres, Elsa, Ricco, Pierre, Lacroix, Damien, Trepat, Xavier, (2015). Traction forces of endothelial cells under slow shear flow Biophysical Journal 1098, 1533-1536
Serra-Picamal, Xavier, Conte, Vito, Sunyer, Raimon, Muñoz, José J., Trepat, Xavier, (2015). Mapping forces and kinematics during collective cell migration Academic Press 125, 309-330
Vedula, S. R. K., Hirata, H., Nai, M. H., Brugués, A., Toyama, Y., Trepat, X., Lim, C. T., Ladoux, B., (2014). Epithelial bridges maintain tissue integrity during collective cell migration Nature Materials 131, 87-96
Elosegui, A., Bazellières, E., Allen, M. D., Andreu, I., Oria, R., Sunyer, R., Gomm, J. J., Marshall, J. F., Jones, J. L., Trepat, X., Roca-Cusachs, P., (2014). Rigidity sensing and adaptation through regulation of integrin types Nature Materials 136, 631-637
Brugués, A., Anon, E., Conte, V., Veldhuis, J. H., Gupta, M., Colombelli, J., Muñoz, J. J., Brodland, G. W., Ladoux, B., Trepat, X., (2014). Forces driving epithelial wound healing Nature Physics 109, 683–690
Vedula, Sri Ram Krishna, Ravasio, Andrea, Anon, Ester, Chen, Tianchi, Peyret, G., Ashraf, Mohammed, Ladoux, Benoit, (2014). Microfabricated environments to study collective cell behaviors Academic Press 120, 235-252
Kim, Jae Hun, Serra-Picamal, Xavier, Tambe, Dhananjay T., Zhou, Enhua H., Park, Chan Young, Sadati, Monirosadat, Park, Jin-Ah, Krishnan, Ramaswamy, Gweon, Bomi, Millet, Emil, Butler, James P., Trepat, Xavier, Fredberg, Jeffrey J., (2013). Propulsion and navigation within the advancing monolayer sheet Nature Materials 129, 856-863
Theveneau, E., Steventon, B., Scarpa, E., Garcia, S., Trepat, X., Streit, A., Mayor, R., (2013). Chase-and-run between adjacent cell populations promotes directional collective migration Nature Cell Biology 157, 763-772
Roca-Cusachs, P., Sunyer, R., Trepat, X., (2013). Mechanical guidance of cell migration: lessons from chemotaxis Current Opinion in Cell Biology 255, 543-549
Chen, Zaozao, Lessey, Elizabeth, Berginski, Matthew E., Cao, Li, Li, Jonathan, Trepat, Xavier, Itano, Michelle, Gomez, Shawn M., Kapustina, Maryna, Huang, Cai, Burridge, Keith, Truskey, George, Jacobson, Ken, (2013). Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration PLoS ONE 81, e52233
Tambe, D. T., Croutelle, U., Trepat, X., Park, C. Y., Kim, J. H., Millet, E., Butler, J. P., Fredberg, J. J., (2013). Monolayer stress microscopy: Limitations, artifacts, and accuracy of recovered intercellular stresses PLoS ONE 82, e55172
Muñoz, J. J., Conte, V., Asadipour, N., Miodownik, M., (2013). A truss element for modelling reversible softening in living tissues Mechanics Research Communications 49, 44-49
Serra-Picamal, Xavier, Conte, Vito, Vincent, Romaric, Anon, Ester, Tambe, Dhananjay T., Bazellieres, Elsa, Butler, James P., Fredberg, Jeffrey J., Trepat, Xavier, (2012). Mechanical waves during tissue expansion Nature Physics Nature Publishing Group 88, 628-634
Anon, Ester, Serra-Picamal, Xavier, Hersen, Pascal, Gauthier, Nils C., Sheetz, Michael P., Trepat, Xavier, Ladoux, Benoît, (2012). Cell crawling mediates collective cell migration to close undamaged epithelial gaps Proceedings of the National Academy of Sciences of the United States of America 10927, 10891-10896
Nocentini, S., Reginensi, D., Garcia, S., Carulla, P., Moreno-Flores, Wandosell, F., Trepat, X., Bribian, A., Del Rí, (2012). Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and traction force microscopy Cellular and Molecular Life Sciences 6910, 1689-1703
Conte, Vito, Ulrich, Florian, Baum, Buzz, Muñoz, Jose, Veldhuis, Jim, Brodland, Wayne, Miodownik, Mark, (2012). A biomechanical analysis of ventral furrow formation in the Drosophila Melanogaster Embryo PLoS ONE Public Library of Science 74, e34473
Trepat, Xavier, Chen, Zaozao, Jacobson, Ken, (2012). Cell Migration John Wiley & Sons, Inc. 2, 2369–2392
Trepat, X., (2011). Forcing tumor arrest Physics 4, 85
Tambe, Dhananjay T., Corey Hardin, C., Angelini, Thomas E., Rajendran, Kavitha, Park, Chan Young, Serra-Picamal, Xavier, Zhou, Enhua H., Zaman, Muhammad H., Butler, James P., Weitz, David A., Fredberg, Jeffrey J., Trepat, X., (2011). Collective cell guidance by cooperative intercellular forces Nature Materials 106, 469-475
Trepat, X., Fredberg, J. J., (2011). Plithotaxis and emergent dynamics in collective cellular migration Trends in Cell Biology 2111, 638-646
Angelini, Thomas E., Hannezo, Edouard, Trepat, Xavier, Marquez, Manuel, Fredberg, Jeffrey J., Weitz, David A., (2011). Glass-like dynamics of collective cell migration Proceedings of the National Academy of Sciences 10812, 4714-4719
Krishnan, Ramaswamy, Klumpers, Darinka D., Park, Chan Y., Rajendran, Kavitha, Trepat, Xavier, van Bezu, Jan, van Hinsbergh, Victor W. M., Carman, Christopher V., Brain, Joseph D., Fredberg, Jeffrey J., Butler, James P., van Nieuw Amerongen, Geerten P., (2011). Substrate stiffening promotes endothelial monolayer disruption through enhanced physical forces American Journal of Physiology - Cell Physiology 3001, C146-C154
- Soft Lithography
- Micro/Nano fabrication
- Cell stretching
- Live Confocal Microcopy
- Magnetic Tweezers
- Magnetic Twisting Cytometry
- Monolayer stress microscopy
- Traction microscopy
- Julien Colombelli
Institute for Research in Biomedicine, Barcelona
- Eduard Batlle
Institute for Research in Biomedicine, Barcelona
- Roger Guimerà
Universitat Rovira i Virgili, Tarragona
- Roberto Mayor
University College London (UK)
- Erik Sahai
Cancer Research (UK)
- Joaquim Gea
Hospital del Mar, Barcelona
- Benoit Ladoux
Université Paris 7, France
- Jim Butler
- Prof. J. J. Fredberg
Physiology Program, School of Public Health, Harvard University, Boston, USA