Gabriel Gomila Lluch | Group Leader
Lázaro René Izquierdo Fábregas | Postdoctoral Researcher
Martí Checa Nualart | PhD Student
Helena Lozano Caballero | PhD Student
Marc Van der Hofstadt Serrano | PhD Student
Rubén Millán Solsona | Laboratory Technician
Dr. Laura Fumagalli | Senior Researcher
Now: School of Physics and Astronomy – Condensed Matter Physics, University of Manchester
Dr. Annalisa Calò | Postdoc
Now: Postdoc at the Institut Catala de Nanociencia i Nanotecnologia (ICN2)
Dr. Aurora Dols-Pérez | Postdoc
Now: Postdoc at the Institut de Quimica Avançada de Catalunya (CSIC)
Dr. Martin Edwards | Postdoc
Now: Postdoc, University of Utah, Salt Lake City
Daniel Esteban Ferrer | PhD Student
Georg Gramse | PhD Student
Now: Marie Curie postdoc, Johannes Kepler University, Linz, Austria
Dr. Jordi Otero | Postdoc
The main goal of the Nanoscale bioelectrical characterization group is to develop new experimental setups based on atomic force microscopy and theoretical frameworks enabling the measurement of the electrical properties of biological samples at the nanoscale (for example, biomembranes, single viruses or single bacteria).
Above: Topography and dielectric image (capacitance gradient) of a bacteriorhodopsin monolayer patch, 5.3 nm thick on a mica substrate. By combining information from the two images the dielectric constant of the protein layer can be determined with sub-50 nm lateral spatial resolution.
Our main objective is to contribute to develop new label-free biological characterization methods and new electronic biosensors.
During 2015 we have measured the electric polarizability of the main components of the cell membrane – namely lipids, sterols and proteins – with a spatial resolution down to 50 nm. To achieve it we pushed forward the limits of a nanoscale dielectric microscopy technique based on Electrostatic Force Microscopy and developed over the years by our group. Quantifying the response of membrane’s electrical dipoles to electric fields is essential in understanding fundamental bioelectric phenomena such the exchange of ions between the cell and the environment, the formation of electric potentials that can propagate over long distances or the cell response to externally applied electrical fields.
Above right: Electrical potential distribution corresponding to the electric interaction between a voltage biased sharp conducting tip of radius 250 nm and a single bacterial cell. The bacterial cell is represented as a 3D ellipsoid structure with uniform electric polarization. From the calculated electric potential distribution the tip-bacteria capacitance can be calculated and compared to experimental measurements obtained with the Scanning Microwave Microscope, in order to determine the electric permittivity of a single bacteria cell at GHz frequencies.
We have also determined, for the first time, the electromagnetic properties of single bacteria cells in the high frequency range (> GHz) with the use of the Scanning Microwave Microscope and of specific 3D simulation models. We showed that with this approach one can detect the presence of small-scale nanostructures inside microorganisms, providing endless applications in the label-free imaging of single bacterial cells at high spatial resolution. Finally, the group continued its efforts towards revealing nanoscale phenomena in living cells. In particular, we optimized the use of a recently developed Atomic Force Microscope imaging mode (dynamic jumping mode) to image with nanoscale spatial resolution single bacterial cell growth and division on planar supports.
Below: Atomic Force Microscopy topography image of living Enetero Agregative E. Coli bacterial cells on a gelatin coated mica substrate in HEPES buffer solution. The image has been obtained in dynamic jumping mode.
Researchers at IBEC and their collaborators from the Johannes Kepler University of Linz, The University of Manchester and the company Keysight Technologies have now achieved an elusive goal: to measure the electromagnetic properties of biological materials at the level of a single bacterial cell and at very high frequencies (gigahertz).
Having measured the electric polarizability of DNA – a fundamental property that directly influences its biological functions – for the first time ever last year, IBEC´s Nanoscale Bioelectrical Characterization group has made a further breakthrough in the understanding of the dielectric properties of cell constituents by measuring the electric polarizability of the main components of the cell membrane – namely lipids, sterols and proteins – with a spatial resolution down to 50nm.
Gabriel Gomila, IBEC group leader and Associate Professor at the UB, has received an ICREA Academia Prize 2014 for excellence in research and capacity for leadership.
Two groups working together at IBEC demonstrate the potential of electrical studies of single bacterial cells in a paper published in ACS Nano. Gabriel Gomila’s Nanoscale Bioelectrical Characterization group and that of Antonio Juárez, Microbial Biotechnology and Host-pathogen Interaction, combined their expertise on microscopic electrical measurements and bacteria respectively to come up with a way to study the response to external electrical fields of just a single bacterial cell.
The electric polarizability of DNA is a fundamental property that directly influences its biological functions. Despite the importance of this property, however, its measurement has remained elusive so far. In a study published in PNAS today, researchers at Barcelona’s Institute for Bioengineering of Catalonia (IBEC) led by Laura Fumagalli, senior researcher at IBEC and lecturer at the University of Barcelona, and their collaborators at the Institute for Research in Biomedicine (IRB) and at Barcelona Supercomputing Center (BSC), and at Centro Nacional de Biotecnologia (CNB-CSIC) and IMDEA Nanociencia in Madrid, describe how they have found a way to directly measure DNA electric polarizability – represented by its dielectric constant, which indicates how a material reacts to an applied electric field – for the first time ever.
The latest article published by IBEC’s Nanoscale bioelectrical characterization group has made the cover of the journal Nanotechnology.
A new European Marie Curie Initial Training Network involving IBEC’s Nanoscale Bioelectrical Characterization group will attempt to bring research into microwaves – which are extensively used in a host of applications such as telecommunications, microwave ovens and radar – to a whole new level.
Scientists at IBEC in Barcelona have found a way of effectively identifying nanoscale objects and viruses that could offer a breakthrough for biomedical diagnostics, environmental protection and nano-electronics.
IBEC’s Nanoscale Bioelectrical Characterization group, headed by Gabriel Gomila, is a partner in a new EU-funded collaborative project set to develop a new tool for non-destructive 3D nanoscale structural characterization, the Volumetric Scanning Microwave Microscope (VSMM).
Gabriel Gomila and Laura Fumagalli, from the Nanoscale bioelectrical characterization line at IBEC, are two of the authors of the study.
|NANOMICROWAVE Microwave Nanotechnology for Semiconductor and Life Sciences||MARIE CURIE – ITN||Gabriel Gomila|
|V-SMMART Nano Volumetric Scanning Microwave Microscopy Analytical and Research Tool for Nanotechnology||NMP – SME||Gabriel Gomila|
|AFM4NanoMed&Bio European network on applications of Atomic Force Microscopy to Nanomedicine and Life Sciences||EU COST Action TD1002||Gabriel Gomila (Management Committee Substitute Member)|
|NANOELECTOMOGRAPHY Electrical nanotomography based on scanning probe microscopy for nanomaterials and biological samples||MICINN (TEC2013-48344-C2-1-P)||Gabriel Gomila|
Biagi, Maria Chiara, Fabregas, Rene, Gramse, Georg, Van Der Hofstadt, Marc, Juárez, Antonio, Kienberger, Ferry, Fumagalli, Laura, Gomila, Gabriel, (2016). Nanoscale electric permittivity of single bacterial cells at gigahertz frequencies by scanning microwave microscopy ACS Nano 101, 280-288
Van Der Hofstadt, M., Fabregas, R., Biagi, M.C., Fumagalli, L., Gomila. G., (2016). Nanoscale dielectric microscopy of non-planar samples by lift-mode electrostatic force microscopy Nanotechnology 2740, 405706
Dols-Perez, Aurora, Gramse, Georg, Calo, Annalisa, Gomila, Gabriel, Fumagalli, Laura, (2015). Nanoscale electric polarizability of ultrathin biolayers on insulator substrates by electrostatic force microscopy Nanoscale 7, 18327-18336
Van Der Hofstadt, M., Hüttener, M., Juárez, A., Gomila, G., (2015). Nanoscale imaging of the growth and division of bacterial cells on planar substrates with the atomic force microscope Ultramicroscopy 154, 29-36
Botaya, Luis, Otero, Jorge, González, Laura, Coromina, Xavier, Gomila, Gabriel, Puig-Vidal, Manel, (2015). Quartz tuning fork-based conductive atomic force microscope with glue-free solid metallic tips Sensors and Actuators A: Physical 232, 259-266
Esteban-Ferrer, Daniel, Edwards, Martin Andrew, Fumagalli, Laura, Juarez, Antonio, Gomila, Gabriel, (2014). Electric polarization properties of single bacteria measured with electrostatic force microscopy ACS Nano American Chemical Society 810, 9843–9849
Cuervo, A., Dans, P. D., Carrascosa, J. L., Orozco, M., Gomila, G., Fumagalli, L., (2014). Direct measurement of the dielectric polarization properties of DNA Proceedings of the National Academy of Sciences of the United States of America 11135, E3624-E3630
Caló, A., Reguera, D., Oncins, G., Persuy, M. A., Sanz, G., Lobasso, S., Corcelli, A., Pajot-Augy, E., Gomila, G., (2014). Force measurements on natural membrane nanovesicles reveal a composition-independent, high Young's modulus Nanoscale 64, 2275-2285
Dols-Perez, A., Fumagalli, L., Gomila, G., (2014). Structural and nanomechanical effects of cholesterol in binary and ternary spin-coated single lipid bilayers in dry conditions Colloids and Surfaces B: Biointerfaces 116, 295-302
Gramse, G., Kasper, M., Fumagalli, L., Gomila, G., Hinterdorfer, P., Kienberger, F., (2014). Calibrated complex impedance and permittivity measurements with scanning microwave microscopy Nanotechnology 2514, 145703 (8)
Gomila, G., Gramse, G., Fumagalli, L., (2014). Finite-size effects and analytical modeling of electrostatic force microscopy applied to dielectric films Nanotechnology 2525, 255702 (11)
Fumagalli, L., Edwards, Martin Andrew, Gomila, G., (2014). Quantitative electrostatic force microscopy with sharp silicon tips Nanotechnology 2549, 495701 (9)
Castillo-Fernandez, O., Rodriguez-Trujillo, R., Gomila, G., Samitier, J., (2014). High-speed counting and sizing of cells in an impedance flow microcytometer with compact electronic instrumentation Microfluidics and Nanofluidics 161-2, 91-99
Birhane, Y., Otero, J., Pérez-Murano, F., Fumagalli, L., Gomila, G., Bausells, J., (2014). Batch fabrication of insulated conductive scanning probe microscopy probes with reduced capacitive coupling Microelectronic Engineering 119, 44-47
Caballero, D., Fumagalli, L., Teixidor, F., Samitier, J., Errachid, A., (2013). Directing polypyrrole growth by chemical micropatterns: A study of high-throughput well-ordered arrays of conductive 3D microrings Sensors and Actuators B: Chemical 177, 1003-1009
Gramse, G., Dols-Perez, A., Edwards, M. A., Fumagalli, L., Gomila, G., (2013). Nanoscale measurement of the dielectric constant of supported lipid bilayers in aqueous solutions with electrostatic force microscopy Biophysical Journal 1046, 1257-1262
Gomila, G., Esteban-Ferrer, D., Fumagalli, L., (2013). Quantification of the dielectric constant of single non-spherical nanoparticles from polarization forces: Eccentricity effects Nanotechnology 2450, 505713
Gramse, G., Edwards, M.A., Fumagalli, L., Gomila, G., (2013). Theory of amplitude modulated electrostatic force microscopy for dielectric measurements in liquids at MHz frequencies Nanotechnology 2441, 415709
Dols-Perez, A., Sisquella, X., Fumagalli, L., Gomila, G., (2013). Optical visualization of ultrathin mica flakes on semitransparent gold substrates Nanoscale Research Letters 81, 1-5
Fumagalli, Laura, Esteban-Ferrer, Daniel, Cuervo, Ana, Carrascosa, Jose L., Gomila, Gabriel, (2012). Label-free identification of single dielectric nanoparticles and viruses with ultraweak polarization forces Nature Materials Nature Publishing Group 119, 743-826
Calò, A., Sanmartí-Espinal, M., Iavicoli, P., Persuy, M. A., Pajot-Augy, E., Gomila, G., Samitier, J., (2012). Diffusion-controlled deposition of natural nanovesicles containing G-protein coupled receptors for biosensing platforms Soft Matter 846, 11632-11643
Gramse, G., Gomila, G., Fumagalli, L., (2012). Quantifying the dielectric constant of thick insulators by electrostatic force microscopy: effects of the microscopic parts of the probe Nanotechnology 2320, 205703
Gramse, G., Edwards, M. A., Fumagalli, L., Gomila, G., (2012). Dynamic electrostatic force microscopy in liquid media Applied Physics Letters 10121, 213108
Dols-Perez, Aurora, Fumagalli, Laura, Cohen Simonsen, Adam, Gomila, Gabriel, (2011). Ultrathin spin-coated dioleoylphosphatidylcholine lipid layers in dry conditions: A combined atomic force microscopy and nanomechanical study Langmuir 2721, 13165-13172
Fumagalli, L., Gramse, G., Esteban-Ferrer, D., Edwards, M. A., Gomila, G., (2010). Quantifying the dielectric constant of thick insulators using electrostatic force microscopy Applied Physics Letters 9618, 183107
Toset, J., Gomila, G., (2010). Three-dimensional manipulation of gold nanoparticles with electro-enhanced capillary forces Applied Physics Letters 964, 043117
Sanmarti, M., Iavicoli, P., Pajot-Augy, E., Gomila, G., Samitier, J., (2010). Human olfactory receptors immobilization on a mixed self assembled monolayer for the development of a bioelectronic nose Procedia Engineering (EUROSENSOR XXIV CONFERENCE) Elsevier Science 5, 786-789
Fumagalli, L., Ferrari, G., Sampietro, M., Gomila, G., (2009). Quantitative nanoscale dielectric microscopy of single-layer supported biomembranes Nano Letters 94, 1604-1608
Gramse, G., Casuso, I., Toset, J., Fumagalli, L., Gomila, G., (2009). Quantitative dielectric constant measurement of thin films by DC electrostatic force microscopy Nanotechnology 2039, 395702
Rodriguez-Trujillo, R., Castillo-Fernandez, O., Garrido, M., Arundell, M., Valencia, A., Gomila, G., (2008). High-speed particle detection in a micro-Coulter counter with two-dimensional adjustable aperture Biosensors and Bioelectronics 242, 290-296
Gomila, G., Toset, J., Fumagalli, L., (2008). Nanoscale capacitance microscopy of thin dielectric films Journal of Applied Physics 1042, 8
Rodriguez-Trujillo, R., Castillo-Fernandez, O., Arundell, M., Samitier, J., Gomila, G., (2008). Yeast cells detection in a very fast and highly versatile microfabricated cytometer MicroTAS 2008 Chemical and Biological Microsystems Society , 1888-1890
Casuso, I., Pla, M., Gomila, G., Samitier, J., Minic, J., Persuy, M. A., Salesse, R., Pajot-Augy, E., (2008). Immobilization of olfactory receptors onto gold electrodes for electrical biosensor Materials Science & Engineering C Elsevier Science 285-6, 686-691
- Cypher Atomic Force Microscope (Asylum Research)
- Nanowizard 4 Bio-Atomic Force Microscope (JPK)
- Cervantes Atomic Force Microscope (Nanotec Electronica)
- Easy Scan 2 Atomic Force Microscope (Nanosurf)
- AxioImager A1m Reflection Optical Microscope (Zeiss) equipped with a AxioCam ERc5s (Zeiss)
- CompactStat portable electrochemical interface and impedance analyzer (Ivium Technologies)
- 2 eLockIn204 4-phase Lock-In amplifiers (Anfatec)
- Keithley 6430 sub-femtoAmp remote sourcemeter (Keithley)
- Dra. Laura Fumagalli
University of Manchester, United Kingdom
- Prof. Jose L. Carrascosa
Department of Structure of Macromolecules, Centro Nacional de Biotecnología, Spain
- Dr. Manel Puig
Departament d’Electrònica, University of Barcelona, Spain
- Dr. Ferry Kienberger
Agilent Technologies Austria, Linz, Austria
- Prof. Marco Sampietro
Politecnico di Milano, Italy
- Prof. Joan Bausells
Centro Nacional de Microelectrónica de Barcelona-CSIC, Spain
- Prof. Antonio Juárez
University of Barcelona, Spain