We aim at understanding how physical forces and molecular control modules cooperate to drive biological function.
We develop new technologies to map and perturb the main physical properties that determine how cells and tissues grow, move, invade and remodel. By combining this physical information with systematic molecular perturbations and computational models we explore the principles that govern the interplay between chemical and physical cues in living tissues.
We study how these principles are regulated in physiology and development, and how they are derailed in cancer and aging.
Making cellular forces visible
To study cell and tissue dynamics we develop new technologies to measure physical forces at the cell-cell and cell-matrix interface. By combining these technologies with computational analysis of cell shape and velocity we obtain a full experimental characterization of epithelial dynamics during tissue growth, wound healing and cancer cell invasion.
Tumour invasion by stromal forces
Cancer cell invasion and metastasis remain the leading cause of death in patients with cancer. Both processes are the result of a complex interaction between tumor cells and their microenvironment. One of our main lines of research is to study how tumours exploit the functions non-cancer cells in their microenvironment to invade and metasize. We focus on the interaction between epithelial cancer cells and Cancer Associated Fibroblasts (CAFs), the most abundant cell type in the tumour stroma. In a recent study we were able to demonstrate that CAFs guide the collective invasion of cancer cells through a physical force. This force enables CAFs to physically drag cancer cells into the surrounding tissue. Force transmission is mediated by a heterotypic interaction between two different proteins, one located on the surface of cancer cells called E-cadherin, and another expressed on the surface of fibroblasts, called N-cadherin.
Optogenetics to control cell mechanics
The recent development of optogenetic technologies offers promising possibilities to control signalling pathways with high spatiotemporal resolution. By expressing genetically encoded light-sensitive proteins, optogenetic technology enables the reversible perturbation of intracellular biochemistry with subcellular resolution. We have developed optogenetic tools based on controlling the activity of endogenous RhoA to upregulate or downregulate cell contractility. We have shown that these tools enable rapid, local and reversible changes in traction forces, cell–cell forces, and tissue compaction. We have shown, further, that changes in cellular forces are paralleled by translocation of the transcriptional regulator YAP, indicating that our tools can be used to control mechanotransductory pathways.
Collective durotaxis: a mechanism for cellular guidance by mechanical cues
Directed cell migration is one of the earliest observations in cell biology, dating back to the late XIX century. Also known as taxis, directed cell migration has been commonly associated with chemotaxis, i.e. the ability of a broad variety of cell types to migrate following gradients of chemical factors. We recently demonstrated a new mode of collective cell guidance by mechanical cues, called collective durotaxis. This new migration mode emerges only in cell collectives and, strikingly, does not require isolated cells to exhibit gradient sensing. To study the mechanisms behind this phenomenon, we developed new tools to measure the forces that propel cells during durotaxis at the cell-matrix and cell-cell levels. Upon combining this new experimental technique with biochemical approaches and theoretical modelling, we concluded that collective durotaxis originates from long-range transmission of contractile intercellular forces. This mechanism is unique in that the very same machinery that senses the attractant -the actomyosin cytoskeleton- is responsible for propulsion towards it. As such, collective durotaxis appears to be the simplest and perhaps most primitive mechanism by which a collective system responds to a gradient.
Microfabrication and wound healing
Using microfabrication technologies, we designed new ways to decipher the mechanisms of wound healing. By doing so we uncovered a new understanding of how cells move and work together to close a gap in a tissue. We showed that a new mechanism applies in which cells assemble supracellular contractile arcs that compress the tissue under the wound. By combining experiments and computational modeling, we showed that contractions arising from these arcs make the wound heal in a quicker and more robust way.
Stretching epithelial tissues
Epithelial tissues are fundamental to life, as they protect the body from radiation, pollutants and pathogens. They’re also responsible for gas exchange in the lungs, absorption of nutrients in the gut, and excretion of urine in the kidneys. We developed a new approach to subject epithelial tissues to very large deformations, up to four times their original size. Most materials are ‘unhappy’ during stretching. As they become progressively deformed, they want to go back to their unstretched state, like a rubber band, or may even break as the tension increases. We found that epithelial sheets have a different and unusual mechanical behavior. To our surprise, tissues did not break during stretching, and they were able to recover their initial size in a fully reversible way when unstretched. Even more surprisingly, some cells in the tissue barely stretched, while others became ‘superstretched’, increasing their area more than ten times. We identified the molecular mechanisms that explain this physical behavior, which we call ‘active superelasticity’ as an analogy with the behavior of some high-tech metal alloys used in medical technologies. Understanding this surprising mechanical behavior in epithelial tissues could help us build better artificial organs or new bionic technologies such as organs-on-a-chip.
Xavier Trepat | Group Leader / ICREA Research Professor
Dobryna Julia Valeria Zalvidea | Senior Researcher
Juan Francisco Abenza Martínez | Postdoctoral Researcher
Tom Golde | Postdoctoral Researcher
Manuel Gómez González | Postdoctoral Researcher
Anna Labernadie | Postdoctoral Researcher
Andrea Malandrino | Postdoctoral Researcher
Marija Matejcic | Postdoctoral Researcher
Leone Rossetti | Postdoctoral Researcher
Raimon Sunyer Borrell | Postdoctoral Researcher
Gerardo Ceada Torres | PhD Student
Nimesh Ramesh Chahare | PhD Student
Sefora Conti | PhD Student
Ariadna Marin Llaurado | PhD Student
Macià Esteve Pallares Pallares | PhD Student
Isabela Corina Santos Fortunato | PhD Student
Miquel Bosch Padrós | Masters Student
Alice Perucca | Masters Student
Oriol Mañé Benach | Laboratory Assistant
Jonel Trebicka | Visiting Researcher
An opinion piece by IBEC group leader Xavier Trepat has appeared in the News and Views section of the current issue of Nature, which is devoted to ‘Bottom-up biology’.
IBEC researchers were in the limelight today at the awards ceremony for the “la Caixa” fellowships and grants for research and innovation calls.
Wednesday’s press release about the Nature paper published by IBEC and UPC researchers got a lot of press coverage.
One of the most enviable features of superheroes is their ability to stretch their bodies beyond imaginable limits. In a study published today in Nature, scientists have discovered that our cells can do just that.
The embryonic stem cells that form faces – neural crest cells – use an unexpected mechanism to develop our facial features, according to a new UCL-led study involving IBEC researchers.
IBEC group leader Xavier Trepat penned a short article for ARA magazine in which he discusses the research of Arthur Ashkin, Gérard Mourou and Donna Strickland, winners of the the Nobel Prize for Physics.
Researchers from IBEC and UB have discovered that the way tumor cells expand defies the laws of physics.
Today IBEC welcomed the new Director General of Research, Development, and Innovation of the Spanish Ministry of Science, Innovation and Universities (MINECO) to meet some of the group leaders and hear about their research.
A review by IBEC group leader and ICREA research professor Xavier Trepat is one of six featured in Nature Physics’ latest ‘Insight’ issue, ‘The Physics of Living Systems’, in which all the articles have been co-authored by a physicist and a biologist.
In a La Vanguardia feature by Josep Corbella, IBEC’s Xavier Trepat is interviewed along with IRB’s Salvador Aznar-Benitah about the important role music plays in their lives, how it has affected their careers as researchers, and about the relation and similarities between musical and scientific creativity.
An article by IBEC researchers Pilar Rodríguez, Xavier Trepat and Raimon Sunyer about the importance of physical forces in understanding biological function appears in the June edition of Investigación y Ciencia, the Spanish-language version of Scientific American magazine.
Researchers at IBEC have discovered that cell division in epithelial tissues is regulated by mechanical forces.
IBEC group leader and ICREA research professor Xavier Trepat has been elected as a Member of EMBO, the European Molecular Biology Organisation.
Alberto Elosegui-Artola, Xavier Trepat and Pere Roca-Cusachs’ paper in Trends in Cell Biology has made the cover of the latest issue of the Cell-family journal.
Researchers at IBEC and MIT have shown that cells could use their environment to mechanically communicate with each other within tissues. It’s a bit like when an army cadet pulls some rope netting taut so that his friend can safely ascend.
IBEC is the recipient of one of twelve MIT-Spain ”la Caixa” Foundation Seed Fund grants under a brand new funding programme in collaboration with the Massachusetts Institute of Technology.
Researchers have shown for the first time that ion channels that are capable of detecting changes in the physical properties of the cellular environment play a key role in tumor invasion and metastasis.
The way cells find their way around is by ‘groping’ rather than seeing their surroundings: this is the main conclusion of a study published in Nature last week involving several IBEC groups and their collaborators.
In their effort to shed light on the role that physical forces play in the body, Pere Roca-Cusachs’ group at IBEC has shown how these forces ‘switch on’ the expression of genes that may result in cancer.
Researchers at the Institute for Bioengineering of Catalonia (IBEC) have observed, for the first time, mechanical waves that form after collisions between cellular tissues.
A video produced by Fundación Banco Sabadell of Xavier Trepat, 2015 winner of the Banco Sabadell Award for Biomedical Research, appears on their YouTube channel.
Three IBEC group leaders – Pere Roca-Cusachs, Vito Conte and Xavier Trepat – consolidate the institute’s leadership in mechanobiology by publishing a review of the field in Nature Cell Biology.
IBEC has signed a Memorandum of Understanding with the EF Clif, the European Foundation for the Study of Chronic Liver Failure.
In a study published today in Nature Cell Biology and supported by Obra Social “la Caixa”, researchers at IBEC have identified an interaction between two proteins that enables cancerous cells to use the physical forces of healthy cells to start tumor metastasis.
Researchers at IBEC have controlled the contractility of a group of epithelial cells using an optogenetic switch activated by light.
A new phenomenon, collective durotaxis, opens new avenues to control tumor growth and improve wound healing
Xavier Trepat, ICREA professor and group leader at IBEC, was the guest star at the first anniversary celebration of Big Vang, La Vanguardia’s online science section.
Researchers at IBEC and their collaborators at the Centre of Regenerative Medicine of Barcelona (CMR[B]) have developed a revolutionary new technique based on photoactivation (light activation), by which cells in deep tissue can activated and tracked in vivo without causing any damage.
IBEC group leader and ICREA research professor Xavier Trepat has come third in the La Vanguardia Science Award.
Scientists at the Institute for Bioengineering of Catalonia (IBEC) have revealed that, counter to previous understanding, the living cells in our bodies behave like solids rather than the liquids they are made of.
A study by the Institute for Bioengineering of Catalonia (IBEC) reveals how cells withstand breakage during the constant changes in shape and volume experienced in most biological processes.
IBEC group leader and ICREA research professor Xavier Trepat is this year’s winner of the Banc Sabadell Award for Biomedical Research for his work on understanding the fundamental biophysical mechanisms underlying cell interaction and communication.
At a press conference at the Obra Social “la Caixa”’s Palau Macaya earlier today, Xavier Trepat, group leader at the Institute for Bioengineering of Catalonia (IBEC), Enric Banda, director of the department of Science and Environment of the Obra Social ”la Caixa”, and Josep Samitier, director of IBEC, described a study published in Nature Cell Biology which sheds new light on how to control metastasis.
Researchers at the Institute for Bioengineering of Catalonia and their collaborators reveal that they’re a step closer to optimizing cells able to guide regeneration of the spine
In an article published in the journal Nature Materials, researchers at the IBEC and the UPC describe their discovery that ‘fracking’takes place in the body at a cellular level.
Researchers at IBEC reveal in a Nature Communications paper some surprising mechanics that drive epithelial gap closure in the absence of underlying layers.
Xavier Serra Picamal, formerly a PhD student in IBEC’s Integrative Cell and Tissue Dynamics group, was awarded a prestigious Ramon Margalef Prize for the best paper derived from a doctoral thesis at a ceremony at the UB last night.
Today’s news about the Integrative Cell and Tissue Dynamics group’s paper in Nature Physics has been covered in La Vanguardia.
When we think of wound healing, we normally think of wounds to our skin. But wounds happen inside the body in all sorts of tissues and organs, and can have implications in many chronic diseases such as diabetes and asthma. Wounds also favour cancer progression by providing a physical and chemical environment that promotes the invasion of malignant cells. Now, a group at the Institute for Bioengineering of Catalonia (IBEC) has found a new way to decipher the mechanisms of wound healing, and by doing so has uncovered a new understanding of how cells move and work together to close a gap in a tissue.
Integrative cell and tissue dynamics group leader Xavier Trepat is profiled in El Mundo’s ‘Innovadores’ supplement today.
The news about Xavier Trepat’s Proof of Concept grant from the ERC appears in La Vanguardia today.
This week’s press release about the Integrative Cell and Tissue Dynamics group’s work published in Nature Materials has appeared online in La Vanguardia, ABC and El Diario.
The Integrative Cell and Tissue Dynamics group starts the year with good news from the European Research Council – they’ve been awarded both a new Consolidator Grant and a Proof of Concept award.
Results from IBEC researchers and their collaborators will pave the way for better and faster wound healing, as well opening new avenues for tissue engineering of skin
The October cover of Current Opinion in Cell Biology (the reference opinion journal in cell biology, with impact factor 12) features research by IBEC’s Pere Roca and Xavier Trepat, who together with Raimon Sunyer from the UB have published a review paper in the issue, “Mechanical guidance of cell migration: lessons from chemotaxis”.
The latest cover of Nature Materials was designed by IBEC PhD student Xavier Serra and shows a real tissue segmented computationally to give the impression of a Gaudinian trencadís.
Integrative Cell and Tissue Dynamics group leader Xavier Trepat and his recent paper in Nature Cell Biology are the subject of an article in El Périodico today.
A new discovery about how cells move inside the body may provide scientists with crucial information about disease mechanisms such as the spread of cancer or the constriction of airways caused by asthma. Researchers at IBEC and Harvard School of Public Health have found that epithelial cells—the type that form a barrier between the inside and the outside of the body, such as skin cells—move in a group, propelled by forces both from within and from nearby cells to fill any spaces they encounter.
Monday’s news about the Nature Cell Biology paper ‘Chase-and-run between adjacent cell populations promotes directional collective migration’ was covered in several science and general news sites and magazines, including La Razón.
Researchers at IBEC, the University of Barcelona and their collaborators have found that cells in our bodies, when moving collectively, carry out something similar to a game of ‘tag’ to coordinate their movement in a particular direction.
Researchers have shed new light on how the cells in our bodies collectively migrate, a critical process in positive events such as embryonic development and wound healing, but which is also integral to the development of cancer.
The Integrative Cell and Tissue Dynamics group’s recent paper published in PNAS was covered in Diario Medico.
First measurements of forces driving collective cell migration unveil new principle in biology
People can be brittle, transparent, shattered, or have a heart of glass. Now these attributes seem all the more appropriate following a discovery by researchers that migrating cells in our bodies behave in a remarkably similar way to glass when it is heated and cooled.
An article by Dr. Xavier Trepat, senior researcher of IBEC´s Cellular and respiratory biomechanics group and the Department of Physiology Sciences of the University of Barcelona, contributes for the first time an experimental answer to the question of how cells move during biological processes as diverse as the development, metastasis, or regeneration of tissues.
Press coverage elsewhere
Cells flow like glass, study finds. Harvard Science Foundation
Cells guided on their journey. Nature Physics
Disorderly conduct. Harvard Medical School Focus
How growing cells move together. Harvard Science Foundation
A stretch in cells. Nature
More than lip service. Nature
In Catalan / Spanish:
Estamos hechos de vidrio. El Mundo
L’observador. RTVE, Spanish National Radio
Com actuen les forces físiques durant la migració cel·lular? Comunicacions UB
|TensionControl Multiscale regulation of epithelial tension (2015-2019)||European Commission, ERC – CoG||Xavier Trepat|
|CAMVAS Coordination and migration of cells during 3D Vasculogenesis (2014-2017)||European Commission, MARIE CURIE – IOF||Xavier Trepat|
|DUROTAXIS Mecanobiología de la durotaxis: de las células aisladas a los tejidos||MINECO, Proyectos I+D Excelencia||Xavier Trepat|
|Joint Programme Healthy Ageing||Obra Social La Caixa||Xavier Trepat|
|Understanding and measuring mechanical tumor properties to improve cancer diagnosis, treatment, and survival: Application to liquid biopsies (2017-2020)||Obra Social La Caixa||Xavier Trepat|
|El mecanoma de la adhesión epitelial: mecanismos de detección, resistencia y transmisión de fuerzas intercelulares||MINECO, I+D-Investigación fundamental no orientada||Xavier Trepat|
|MICROGRADIENTPAGE Micro Gradient Polyacrylamide Gels for High Throughput Electrophoresis Analysis||European Commission, ERC-PoC||Xavier Trepat|
|GENESFORCEMOTION Physical Forces Driving Collective Cell Migration: from Genes to Mechanism||European Commission, ERC-StG||Xavier Trepat|
Pérez-González, Carlos, Alert, Ricard, Blanch-Mercader, Carles, Gómez-González, Manuel, Kolodziej, Tomasz, Bazellieres, Elsa, Casademunt, Jaume, Trepat, Xavier, (2019). Active wetting of epithelial tissues Nature Physics 15, 79-88
Malandrino, Andrea, Trepat, Xavier, Kamm, Roger D., Mak, Michael, (2019). Dynamic filopodial forces induce accumulation, damage, and plastic remodeling of 3D extracellular matrices PLoS Computational Biology PLOS Computational Biology , 15, (4), e1006684
Arroyo, M., Trepat, X., (2019). Embryonic self-fracking Science 365, (6452), 442-443
Park, D., Wershof, E., Boeing, S., Labernadie, A., Jenkins, R. P., George, S., Trepat, X., Bates, P. A., Sahai, E., (2019). Extracellular matrix anisotropy is determined by TFAP2C-dependent regulation of cell collisions Nature Materials Article in Press
Garreta, Elena, Prado, Patricia, Tarantino, Carolina, Oria, Roger, Fanlo, Lucia, Martí, Elisa, Zalvidea, Dobryna, Trepat, Xavier, Roca-Cusachs, Pere, Gavaldà -Navarro, Aleix, Cozzuto, Luca, Campistol, Josep M., Izpisúa Belmonte, Juan Carlos, Hurtado del Pozo, Carmen, Montserrat, Nuria, (2019). Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 18, 397-405
Chen, Tianchi, Callan-Jones, Andrew, Fedorov, Eduard, Ravasio, Andrea, Brugués, Agustí, Ong, Hui Ting, Toyama, Yusuke, Low, Boon Chuan, Trepat, Xavier, Shemesh, Tom, Voituriez, Raphaël, Ladoux, Benoît, (2019). Large-scale curvature sensing by directional actin flow drives cellular migration mode switching Nature Physics 15, (4), 393-402
Uroz, Marina, Garcia-Puig, Anna, Tekeli, Isil, Elosegui-Artola, Alberto, Abenza, Juan F., Marín-Llauradó, Ariadna, Pujals, Silvia, Conte, Vito, Albertazzi, Lorenzo, Roca-Cusachs, Pere, Raya, Ángel, Trepat, Xavier, (2019). Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium Nature Materials 18, 1015-1023
Zalvidea, D., Trepat, X., Rebollo, E., (2019). Fast multiphoton microscope based on polymer ulenses array using a 3D printed mold CL Poster session 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference , IEEE (Munich, Germany) OSA Technical Digest (Optical Society of America, 2019), paper cl_p_22
Zalvidea, D., Castano, O., Baker, S., Castro, N., Engel, E., Trepat, X., (2019). Time-lapse intravital imaging of biomaterials integration in tissues using a multicolor multiphoton microscope Novel lasers, instruments and technology 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference , IEEE (Munich, Germany) OSA Technical Digest (Optical Society of America, 2019), paper cl_3_1
Timmers, H., Kowligy, A., Lind, A. J., Nader, N., Shaw, J., Zalvidea, D., Biegert, J., Diddams, S. A., (2019). Hyperspectral microscopy with broadband infrared frequency combs Ultrafast Applications (SF1E) 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference , IEEE (Munich, Germany) OSA Technical Digest (Optical Society of America, 2019), paper SF1E.4
Latorre, Ernest, Kale, Sohan, Casares, Laura, Gómez-González, Manuel, Uroz, Marina, Valon, Léo, Nair, Roshna V., Garreta, Elena, Montserrat, Nuria, del Campo, Aránzazu, Ladoux, Benoit, Arroyo, Marino, Trepat, Xavier, (2018). Active superelasticity in three-dimensional epithelia of controlled shape Nature 563, (7730), 203-208
Good, M., Trepat, X., (2018). Cell parts to complex processes, from the bottom up Nature 563, (7730), 188-189
Shellard, Adam, Szabó, A., Trepat, Xavier, Mayor, Roberto, (2018). Supracellular contraction at the rear of neural crest cell groups drives collective chemotaxis Science 362, (6412), 339-343
Trepat, Xavier, Sahai, Erik, (2018). Mesoscale physical principles of collective cell organization Nature Physics 14, (7), 671-682
Uroz, Marina, Wistorf, Sabrina, Serra-Picamal, Xavier, Conte, Vito, Sales-Pardo, Marta, Roca-Cusachs, Pere, Guimerà, Roger, Trepat, Xavier, (2018). Regulation of cell cycle progression by cell–cell and cell–matrix forces Nature Cell Biology 20, (6), 646-654
Elosegui-Artola, Alberto, Trepat, Xavier, Roca-Cusachs, Pere, (2018). Control of mechanotransduction by molecular clutch dynamics Trends in Cell Biology 28, (5), 356-367
Thottacherry, Joseph Jose, Kosmalska, Anita Joanna, Elosegui-Artola, Alberto, Pradhan, Susav, Sharma, Sumit, Singh, Parvinder P., Guadamillas, Marta C., Chaudhary, Natasha, Vishwakarma, Ram, Trepat, Xavier, del Pozo, Miguel A., Parton, Robert G., Pullarkat, Pramod, Roca-Cusachs, Pere, Mayor, Satyajit, (2018). Mechanochemical feedback and control of endocytosis and membrane tension Nature Communications 9, 4217
Pardo-Pastor, Carlos, Rubio-Moscardo, Fanny, Vogel-González, Marina, Serra, Selma A., Afthinos, Alexandros, Mrkonjic, Sanela, Destaing, Olivier, Abenza, Juan F., Fernández-Fernández, José M., Trepat, Xavier, Albiges-Rizo, Corinne, Konstantopoulos, Konstantinos, Valverde, Miguel A., (2018). Piezo2 channel regulates RhoA and actin cytoskeleton to promote cell mechanobiological responses Proceedings of the National Academy of Sciences of the United States of America 115, (8), 1925-1930
Dix, Christina L., Matthews, Helen K., Uroz, Marina, McLaren, Susannah, Wolf, Lucie, Heatley, Nicholas, Win, Zaw, Almada, Pedro, Henriques, Ricardo, Boutros, Michael, Trepat, Xavier, Baum, Buzz, (2018). The role of mitotic cell-substrate adhesion re-modeling in animal cell division Developmental Cell 45, (1), 132-145
De Pascalis, Chiara, Pérez-González, Carlos, Seetharaman, Shailaja, Boëda, Batiste, Vianay, Benoit, Burute, Mithila, Leduc, Cécile, Borghi, Nicolas, Trepat, Xavier, Etienne-Manneville, Sandrine, (2018). Intermediate filaments control collective migration by restricting traction forces and sustaining cell–cell contacts The Journal of Cell Biology 217, (9), 3031-3044
Labernadie, Anna, Trepat, Xavier, (2018). Sticking, steering, squeezing and shearing: cell movements driven by heterotypic mechanical forces Current Opinion in Cell Biology 54, 57-65
Sehgal, Poonam, Kong, Xinyu, Wu, Jun, Sunyer, Raimon, Trepat, Xavier, Leckband, Deborah, (2018). Epidermal growth factor receptor and integrins control force-dependent vinculin recruitment to E-cadherin junctions Journal of Cell Science 131, (6), jcs206656
Kuipers, Arthur J., Middelbeek, Jeroen, Vrenken, Kirsten, Pérez-González, Carlos, Poelmans, Geert, Klarenbeek, Jeffrey, Jalink, Kees, Trepat, Xavier, van Leeuwen, Frank N., (2018). TRPM7 controls mesenchymal features of breast cancer cells by tensional regulation of SOX4 Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1864, (7), 2409-2419
Escribano, Jorge, Sunyer, Raimon, Sánchez, María Teresa, Trepat, Xavier, Roca-Cusachs, Pere, García-Aznar, José Manuel, (2018). A hybrid computational model for collective cell durotaxis Biomechanics and Modeling in Mechanobiology 17, (4), 1037-1052
Oria, Roger, Wiegand, Tina, Escribano, Jorge, Elosegui-Artola, Alberto, Uriarte, Juan Jose, Moreno-Pulido, Cristian, Platzman, Ilia, Delcanale, Pietro, Albertazzi, Lorenzo, Navajas, Daniel, Trepat, Xavier, García-Aznar, José Manuel, Cavalcanti-Adam, Elisabetta Ada, Roca-Cusachs, Pere, (2017). Force loading explains spatial sensing of ligands by cells Nature 552, 219-224
Malinverno, C., Corallino, S., Giavazzi, F., Bergert, M., Li, Q., Leoni, M., Disanza, A., Frittoli, E., Oldani, A., Martini, E., Lendenmann, T., Deflorian, G., Beznoussenko, G. V., Poulikakos, D., Ong, K. H., Uroz, M., Trepat, X., Parazzoli, D., Maiuri, P., Yu, W., Ferrari, A., Cerbino, R., Scita, G., (2017). Endocytic reawakening of motility in jammed epithelia Nature Materials 16, 587–596
Rodriguez-Franco, P., Brugués, A., Marin-Llaurado, A., Conte, V., Solanas, G., Batlle, E., Fredberg, J. J., Roca-Cusachs, P., Sunyer, R., Trepat, X., (2017). Long-lived force patterns and deformation waves at repulsive epithelial boundaries Nature Materials 16, (10), 1029-1036
Elosegui-Artola, A., Andreu, I., Beedle, A. E. M., Lezamiz, A., Uroz, M., Kosmalska, A. J., Oria, R., Kechagia, J. Z., Rico-Lastres, P., Le Roux, A. L., Shanahan, C. M., Trepat, X., Navajas, D., Garcia-Manyes, S., Roca-Cusachs, P., (2017). Force triggers YAP nuclear entry by regulating transport across nuclear pores Cell , 171, (6), 1397-1410
Labernadie, A., Kato, T., Brugués, A., Serra-Picamal, X., Derzsi, S., Arwert, E., Weston, A., González-Tarragó, V., Elosegui-Artola, A., Albertazzi, L., Alcaraz, J., Roca-Cusachs, P., Sahai, E., Trepat, X., (2017). A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion Nature Cell Biology 19, (3), 224-237
Roca-Cusachs, Pere, Conte, Vito, Trepat, Xavier, (2017). Quantifying forces in cell biology Nature Cell Biology 19, (7), 742-751
Valon, L., Marín-Llauradó, A., Wyatt, T., Charras, G., Trepat, X., (2017). Optogenetic control of cellular forces and mechanotransduction Nature Communications 8, 14396
Arroyo, M., Trepat, X., (2017). Hydraulic fracturing in cells and tissues: fracking meets cell biology Current Opinion in Cell Biology 44, 1-6
Castellanos, M. I., Mas-Moruno, C., Grau, A., Serra-Picamal, X., Trepat, X., Albericio, F., Joner, M., Gil, F. J., Ginebra, M. P., Manero, J. M., Pegueroles, M., (2017). Functionalization of CoCr surfaces with cell adhesive peptides to promote HUVECs adhesion and proliferation Applied Surface Science , 393, 82-92
Li, Haiyue, Xu, Bin, Zhou, Enhua H., Sunyer, Raimon, Zhang, Yanhang, (2017). Multiscale measurements of the mechanical properties of collagen matrix ACS Biomaterials Science & Engineering 3, (11), 2815-2824
Blanch-Mercader, C., Vincent, R., Bazellières, E., Serra-Picamal, X., Trepat, X., Casademunt, J., (2017). Effective viscosity and dynamics of spreading epithelia: a solvable model Soft Matter 13, (6), 1235-1243
Sunyer, R., Conte, V., Escribano, J., Elosegui-Artola, A., Labernadie, A., Valon, L., Navajas, D., García-Aznar, J. M., Muñoz, J. J., Roca-Cusachs, P., Trepat, X., (2016). Collective cell durotaxis emerges from long-range intercellular force transmission Science 353, (6304), 1157-1161
Ladoux, B., Mège, R. M., Trepat, X., (2016). Front-rear polarization by mechanical cues: From single cells to tissues Trends in Cell Biology 26, (6), 420-433
Tekeli, I., Aujard, I., Trepat, X., Jullien, L., Raya, A., Zalvidea, D., (2016). Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation Light: Science and Applications , 5, (6), e16084
Plutoni, C., Bazellieres, E., Le Borgne-Rochet, M., Comunale, F., Brugues, A., Séveno, M., Planchon, D., Thuault, S., Morin, N., Bodin, S., Trepat, X., Gauthier-Rouvière, C., (2016). P-cadherin promotes collective cell migration via a Cdc42-mediated increase in mechanical forces Journal of Cell Biology , 212, (2), 199-217
Asadipour, N., Trepat, X., Muñoz, J. J., (2016). Porous-based rheological model for tissue fluidisation Journal of the Mechanics and Physics of Solids 96, 535-549
Alencar, A. M., Ferraz, M. S. A., Park, C. Y., Millet, E., Trepat, X., Fredberg, J. J., Butler, J. P., (2016). Non-equilibrium cytoquake dynamics in cytoskeletal remodeling and stabilization Soft Matter 12, (41), 8506-8511
Przybyla, L., Lakins, J. N., Sunyer, R., Trepat, X., Weaver, V. M., (2016). Monitoring developmental force distributions in reconstituted embryonic epithelia Methods , 94, 101-113
Blanchard, R., Morin, C., Malandrino, A., Vella, A., Sant, Z., Hellmich, C., (2016). Patient-specific fracture risk assessment of vertebrae: A multiscale approach coupling X-ray physics and continuum micromechanics International Journal for Numerical Methods in Biomedical Engineering , 32, (9), e02760
Casares, L., Vincent, R., Zalvidea, D., Campillo, N., Navajas, D., Arroyo, M., Trepat, X., (2015). Hydraulic fracture during epithelial stretching Nature Materials 14, (3), 343-351
Bazellières, Elsa, Conte, Vito, Elosegui, Alberto, Serra-Picamal, Xavier, Bintanel-Morcillo, María, Roca-Cusachs, Pere, Muñoz, José J., Sales-Pardo, Marta, Guimerà, Roger, Trepat, Xavier, (2015). Control of cell-cell forces and collective cell dynamics by the intercellular adhesome Nature Cell Biology 17, (4), 409-420
Ravasio, Andrea, Cheddadi, Ibrahim, Chen, Tianchi, Pereira, Telmo, Ong, Hui Ting, Bertocchi, Cristina, Brugues, Agusti, Jacinto, Antonio, Kabla, Alexandre J., Toyama, Yusuke, Trepat, Xavier, Gov, Nir, Neves de Almeida, Luis, Ladoux, Benoit, (2015). Gap geometry dictates epithelial closure efficiency Nature Communications 6, 7683
Vedula, Sri Ram Krishna, Peyret, Grégoire, Cheddadi, Ibrahim, Chen, Tianchi, Brugués, Agustí, Hirata, Hiroaki, Lopez-Menendez, Horacio, Toyama, Yusuke, Neves de Almeida, Luis, Trepat, Xavier, Lim, Chwee Teck, Ladoux, Benoit, (2015). Mechanics of epithelial closure over non-adherent environments Nature Communications 6, 6111
Kosmalska, A. J., Casares, L., Elosegui, A., Thottacherry, J. J., Moreno-Vicente, R., González-Tarragó, V., Del Pozo, M. Ã, Mayor, S., Arroyo, M., Navajas, D., Trepat, X., Gauthier, N. C., Roca-Cusachs, P., (2015). Physical principles of membrane remodelling during cell mechanoadaptation Nature Communications 6, 7292
Vincent, Romaric, Bazellières, Elsa, Pérez-González, Carlos, Uroz, Marina, Serra-Picamal, Xavier, Trepat, Xavier, (2015). Active tensile modulus of an epithelial monolayer Physical Review Letters 115, (24), 248103
Lucantonio, Alessandro, Noselli, Giovanni, Trepat, Xavier, DeSimone, Antonio, Arroyo, Marino, (2015). Hydraulic fracture and toughening of a brittle layer bonded to a hydrogel Physical Review Letters 115, (18), 188105
Brask, J. B., Singla-Buxarrais, G., Uroz, M., Vincent, R., Trepat, X., (2015). Compressed sensing traction force microscopy Acta Biomaterialia 26, 286-294
Reginensi, Diego, Carulla, Patricia, Nocentini, Sara, Seira, Oscar, Serra-Picamal, Xavier, Torres-Espín, Abel, Matamoros-Angles, Andreu, Gavín, Rosalina, Moreno-Flores, María Teresa, Wandosell, Francisco, Samitier, Josep, Trepat, Xavier, Navarro, Xavier, del Río, José Antonio, (2015). Increased migration of olfactory ensheathing cells secreting the Nogo receptor ectodomain over inhibitory substrates and lesioned spinal cord Cellular and Molecular Life Sciences , 72, (14), 2719-2737
García, S., Sunyer, R., Olivares, A., Noailly, J., Atencia, J., Trepat, X., (2015). Generation of stable orthogonal gradients of chemical concentration and substrate stiffness in a microfluidic device Lab on a Chip 15, (12), 2606-2614
Vizoso, Miguel, Puig, Marta, Carmona, F. Javier, Maqueda, Maria, Velásquez, Adriana, Gomez, Antonio, Labernadie, Anna, Lugo, Roberto, Gabasa, Marta, Rigat-Brugarolas, Luis G., Trepat, Xavier, Ramírez, Jose, Reguart, Noemí, Moran, Sebastian, Vidal, Enrique, Perera, Alexandre, Esteller, Manel, Alcaraz, Jordi, (2015). Aberrant DNA methylation in Non Small Cell Lung Cancer associated fibroblasts Carcinogenesis , 32, (12), 1453-1463
Mrkonji, Garcia-Elias, A., Pardo-Pastor, C., Bazellières, E., Trepat, X., Vriens, J., Ghosh, D., Voets, T., Vicente, R., Valverde, M. A., (2015). TRPV4 participates in the establishment of trailing adhesions and directional persistence of migrating cells Pflugers Archiv European Journal of Physiology , 467, (10), 2107-2119
Zaritsky, Assaf, Welf, Erik S., Tseng, Yun-Yu, Angeles Rabadán, M., Serra-Picamal, Xavier, Trepat, Xavier, Danuser, Gaudenz, (2015). Seeds of locally aligned motion and stress coordinate a collective cell migration Biophysical Journal , 109, (12), 2492-2500
Perrault, Cecile, Brugues, Agusti, Bazellieres, Elsa, Ricco, Pierre, Lacroix, Damien, Trepat, Xavier, (2015). Traction forces of endothelial cells under slow shear flow Biophysical Journal , 109, (8), 1533-1536
Serra-Picamal, Xavier, Conte, Vito, Sunyer, Raimon, Muñoz, José J., Trepat, Xavier, (2015). Mapping forces and kinematics during collective cell migration Methods in Cell Biology - Biophysical Methods in Cell Biology (ed. Wilson, L., Tran, P.), Academic Press (Santa Barbara, USA) 125, 309-330
Vedula, S. R. K., Hirata, H., Nai, M. H., Brugués, A., Toyama, Y., Trepat, X., Lim, C. T., Ladoux, B., (2014). Epithelial bridges maintain tissue integrity during collective cell migration Nature Materials 13, (1), 87-96
Elosegui, A., Bazellières, E., Allen, M. D., Andreu, I., Oria, R., Sunyer, R., Gomm, J. J., Marshall, J. F., Jones, J. L., Trepat, X., Roca-Cusachs, P., (2014). Rigidity sensing and adaptation through regulation of integrin types Nature Materials 13, (6), 631-637
Brugués, A., Anon, E., Conte, V., Veldhuis, J. H., Gupta, M., Colombelli, J., Muñoz, J. J., Brodland, G. W., Ladoux, B., Trepat, X., (2014). Forces driving epithelial wound healing Nature Physics 10, (9), 683–690
Trepat, Xavier, (2014). Discussion of “Cytoskeletal Mechanics Regulating Amoeboid Cell Locomotion” (Álvarez-González, B., Bastounis, E., Meili, R., del Alamo, J. C., Firtel, R. A., and Lasheras, J. C., 2014, ASME Appl. Mech. Rev., 66(5), p. 050804) Applied Mechanics Reviews , 66, (5), 055502
Vedula, Sri Ram Krishna, Ravasio, Andrea, Anon, Ester, Chen, Tianchi, Peyret, G., Ashraf, Mohammed, Ladoux, Benoit, (2014). Microfabricated environments to study collective cell behaviors Methods in Cell Biology (ed. Piel, M., Théry, M.), Academic Press 120, 235-252
Kim, Jae Hun, Serra-Picamal, Xavier, Tambe, Dhananjay T., Zhou, Enhua H., Park, Chan Young, Sadati, Monirosadat, Park, Jin-Ah, Krishnan, Ramaswamy, Gweon, Bomi, Millet, Emil, Butler, James P., Trepat, Xavier, Fredberg, Jeffrey J., (2013). Propulsion and navigation within the advancing monolayer sheet Nature Materials 12, (9), 856-863
Theveneau, E., Steventon, B., Scarpa, E., Garcia, S., Trepat, X., Streit, A., Mayor, R., (2013). Chase-and-run between adjacent cell populations promotes directional collective migration Nature Cell Biology 15, (7), 763-772
Roca-Cusachs, P., Sunyer, R., Trepat, X., (2013). Mechanical guidance of cell migration: lessons from chemotaxis Current Opinion in Cell Biology 25, (5), 543-549
Chen, Zaozao, Lessey, Elizabeth, Berginski, Matthew E., Cao, Li, Li, Jonathan, Trepat, Xavier, Itano, Michelle, Gomez, Shawn M., Kapustina, Maryna, Huang, Cai, Burridge, Keith, Truskey, George, Jacobson, Ken, (2013). Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration PLoS ONE 8, (1), e52233
Tambe, D. T., Croutelle, U., Trepat, X., Park, C. Y., Kim, J. H., Millet, E., Butler, J. P., Fredberg, J. J., (2013). Monolayer stress microscopy: Limitations, artifacts, and accuracy of recovered intercellular stresses PLoS ONE 8, (2), e55172
Muñoz, J. J., Conte, V., Asadipour, N., Miodownik, M., (2013). A truss element for modelling reversible softening in living tissues Mechanics Research Communications , 49, 44-49
Serra-Picamal, Xavier, Conte, Vito, Vincent, Romaric, Anon, Ester, Tambe, Dhananjay T., Bazellieres, Elsa, Butler, James P., Fredberg, Jeffrey J., Trepat, Xavier, (2012). Mechanical waves during tissue expansion Nature Physics Nature Publishing Group 8, (8), 628-634
Anon, Ester, Serra-Picamal, Xavier, Hersen, Pascal, Gauthier, Nils C., Sheetz, Michael P., Trepat, Xavier, Ladoux, Benoît, (2012). Cell crawling mediates collective cell migration to close undamaged epithelial gaps Proceedings of the National Academy of Sciences of the United States of America 109, (27), 10891-10896
Nocentini, S., Reginensi, D., Garcia, S., Carulla, P., Moreno-Flores, Wandosell, F., Trepat, X., Bribian, A., Del Rí, (2012). Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and traction force microscopy Cellular and Molecular Life Sciences , 69, (10), 1689-1703
Conte, Vito, Ulrich, Florian, Baum, Buzz, Muñoz, Jose, Veldhuis, Jim, Brodland, Wayne, Miodownik, Mark, (2012). A biomechanical analysis of ventral furrow formation in the Drosophila Melanogaster Embryo PLoS ONE Public Library of Science 7, (4), e34473
Trepat, Xavier, Chen, Zaozao, Jacobson, Ken, (2012). Cell Migration Comprehensive Physiology (ed. Terjung, Ron), John Wiley & Sons, Inc. (Hoboken, USA) 2, 2369–2392
Trepat, X., (2011). Forcing tumor arrest Physics , 4, 85
Tambe, Dhananjay T., Corey Hardin, C., Angelini, Thomas E., Rajendran, Kavitha, Park, Chan Young, Serra-Picamal, Xavier, Zhou, Enhua H., Zaman, Muhammad H., Butler, James P., Weitz, David A., Fredberg, Jeffrey J., Trepat, X., (2011). Collective cell guidance by cooperative intercellular forces Nature Materials 10, (6), 469-475
Trepat, X., Fredberg, J. J., (2011). Plithotaxis and emergent dynamics in collective cellular migration Trends in Cell Biology 21, (11), 638-646
Angelini, Thomas E., Hannezo, Edouard, Trepat, Xavier, Marquez, Manuel, Fredberg, Jeffrey J., Weitz, David A., (2011). Glass-like dynamics of collective cell migration Proceedings of the National Academy of Sciences of the United States of America 108, (12), 4714-4719
Krishnan, Ramaswamy, Klumpers, Darinka D., Park, Chan Y., Rajendran, Kavitha, Trepat, Xavier, van Bezu, Jan, van Hinsbergh, Victor W. M., Carman, Christopher V., Brain, Joseph D., Fredberg, Jeffrey J., Butler, James P., van Nieuw Amerongen, Geerten P., (2011). Substrate stiffening promotes endothelial monolayer disruption through enhanced physical forces American Journal of Physiology - Cell Physiology , 300, (1), C146-C154
- Soft Lithography
- Micro/Nano fabrication
- Cell stretching
- Live Confocal Microcopy
- Magnetic Tweezers
- Magnetic Twisting Cytometry
- Monolayer stress microscopy
- Traction microscopy
- Julien Colombelli / Eduard Batlle
Institute for Research in Biomedicine (IRB) Barcelona
- Marino Arroyo
Universitat Politècnica de Catalunya, Barcelona
- Guillaume Charras / Roberto Mayor
University College London, UK
- Erik Sahai
Cancer Research, UK
- Benoit Ladoux
Université Paris 7, France
- Jim Butler & Jeff Fredberg
Harvard University, Boston
- Danijela Vignjevic
Institut Curie, Paris