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by Keyword: Cryo-TEM


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Manca, M. L., Castangia, I., Matricardi, P., Lampis, S., Fernàndez-Busquets, X., Fadda, A. M., Manconi, M., (2014). Molecular arrangements and interconnected bilayer formation induced by alcohol or polyalcohol in phospholipid vesicles Colloids and Surfaces B: Biointerfaces 117, 360-367

A self-assembled hybrid phospholipid vesicular system containing various penetration enhancers - ethanol, Transcutol and propylenglycol - was prepared and characterized. The effects of the different alcohol or polyalcohols structure and their concentration on the features of the assembled vesicles were evaluated using a combination of different techniques, including cryo-transmission electron microscopy, laser light scattering, differential scanning calorimetry, small- and wide-angle X-ray scattering and rheological analysis. These techniques allow explaining the structural rearrangements of the bilayer assembly due to the alcohol or polyalcohol addition. X-ray scattering studies showed that such addition at the highest concentration (20%) allowed structure modification to oligolamellar vesicles and a bilayer transition to interdigitated phase. Rheological studies confirmed the importance of alcohol or polyalcohol in the structuring dispersions probably due to a partial tilting of phosphatidylcholine acyl chains forming interdigitated and interconnected bilayer vesicles.

Keywords: (Poly)alcohols, Cryo-TEM, DSC, Liposomes, Penetration Enhancer containing Vesicle (PEVs), Rheology, SAXS