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del Rio, Jose Antonio, Soriano, Eduardo, (2010). Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration Nature Protocols 5, (2), 217-226

We present a method for using long-term organotypic slice co-cultures of the entorhino-hippocampal formation to analyze the axon-regenerative properties of a determined compound. The culture method is based on the membrane interphase method, which is easy to perform and is generally reproducible. The degree of axonal regeneration after treatment in lesioned cultures can be seen directly using green fluorescent protein (GFP) transgenic mice or by axon tracing and histological methods. Possible changes in cell morphology after pharmacological treatment can be determined easily by focal in vitro electroporation. The well-preserved cytoarchitectonics in the co-culture facilitate the analysis of identified cells or regenerating axons. The protocol takes up to a month.

Keywords: Cajal-retzius cells, Green-fluorescent-protein, In-vitro model, Rat hippocampus, Nervous-tissue, Brain-slices, Dentate gyrus, Gene-transfer, Cultures, Damage